Hepatitis treatment is one of the most important areas of ozone therapy. In this case, the therapeutic effect of ozone occurs through its direct action on the virus and also through its indirect action with immunomodulatory activity. Ozone therapy is effective in all types of viral hepatitis (A, B, C), especially in chronic forms.

Ozone alters the function of the reverse transcriptase enzyme involved in the manufacture of virus proteins, which prevents the virus from replicating by interfering with the polypeptide chains in the virus membrane that prevent the virus from adhering to target cells (hepatocytes) (Freberg, Carpendale, 1988). Because they contain more lipid, which reacts readily with ozone, encapsulated viruses are more vulnerable to it.

The additional ozone-induced peroxide supply always intensifies the decreased cell phagocytic activity in hepatitis. The ozone-oxygen gas mixture activates both cellular and humoral immunity. Ozone therapy causes an increase in the formation of cytokines, especially interferon, which is one of the most important factors in the body's endogenous defense against viral infection. As a result, there is an increase in the synthesis of T-killer cells, these cells are responsible for humoral immunity and normalization of the formation of T-helper cells that regulate the function of B lymphocytes. These mentioned events ultimately lead to the suppression and elimination of the inflammatory process.

Ozone has a positive effect on hemostasis by decreasing platelet aggregation, preventing the development of a secondary reactive inflammation (microncrosis and microthrombosis), increasing the fibrinoloitic activity and hypocoagulation of the blood. By increasing the elasticity and deformability of erythrocytes, active oxygen increases the oxygen-transport function of erythrocytes and thus microcirculation and oxygenation of tissues. It eliminates the imbalance between oxidative free radical processes and endogenous antioxidant synthesis.

The treatment method of choice in chronic hepatitis is major autohemotherapy with ozone, which has a high activity level feature. In the first MOHT application, 500 mcg ozone dose – 100 ml blood and the same volume of gas mixture 5000 mcg/L (trial application) ozone concentration is used, for later applications the ozone dose is 700 mcg – 100 ml and the ozone concentration in my gas mixture in the same volume is 7000- It is gradually increased to 10000 mcg/L (approximately 6-8 applications every other day). After the decrease in the transaminase level is achieved, the ozone dose is reduced to 700-500 mcg - 100 ml of blood and the ozone concentration in the same volume of gas mixture to 7000-5000 mcg/L (1 application per week) until the obtained antioxidant level is stabilized. In less severe cases of hepatitis, a course of 10-12 days of ozone therapy with intravenous infusion of 400 ml of ozonized serum physiologic at a concentration of 1600-2000 mcg/L is planned.

Another form of treatment is rectal ozone insufflation, which will be performed every other day. 10-15 insufflations are made in a course of treatment. Recommended ozone concentration is 5000-10000 mcg/l in 300-500 ml volume (ozone dose – 1500-3000-5000 mcg). Recently, the rate of toxic hepatitis (caused by alcohol and drugs) has increased considerably among chronic diffuse diseases of the liver. The therapeutic effect of ozone in these cases is due to the formation of peroxides, which initiate the antioxidative mechanism of detoxification of the glutathione system, which acts as a protective sol on the hepatocyte during the activation of lipid peroxidation processes. In the treatment of toxic hepatitis, ozonated saline infusion and rectal insufflation should be used in combination (see above). After 2-3 applications, patients report subjective improvements in their general condition, improvement in appetite and sleep, decrease in skin itching, heaviness and pain in the right precostal region, and dyspepsia, and an increased desire to work.

After a course of treatment, positive improvements in the biochemical and immunological indices of the blood (hyperbilirubinemia, increase in AST, ALT, ALP levels, normalization of albumin stimulating function) occur and viremia disappears in 60% of patients (A.V. Zmyzglova, N.P. Isaeva, 1998). Lipid peroxidation processes are inhibited and at the same time the body's antioxidant defense system is activated. According to rheohepatography and biomicroscopy data, significant improvements are observed in systemic and intrahepatic microcirculation parameters (V.V. Nedogoda, O.Yu. Sviridenko, 2000). It is important that the treatment is well tolerated by patients; No aggression or complications were observed in the patients.

The use of ozone can be extended up to 1-2 months, and applications can be carried out 1-2 times a week. In the treatment of hepatitis, ozone therapy can be used as complementary or monotherapy. It is necessary to use antioxidants together. Hepatitis C and Ozone Treatment HCV is a rapidly increasing disease in the world. The massif has an important place in terms of public health, and its adaptability and pathogenicity are very high. Recognition of the hepatitis virus dates back to the 1970s. Hepatitis C was recognized in 1989. Today, the world human population, migration and travels have rapidly increased the demographic and geographical distribution of the hepatitis C virus. The HCV particle is composed of a nucleocapsid and a single RNA.

It has 9600 nucleotides and has an envelope made of protein. HCV has genotypic flexibility. 60% of the lipid structure is composed of phospholipids and the remainder is cholesterol, and there are about 100 subgroups. Viral life of HCV continues by binding to the receptor on the host cell surface and this is usually hepatocytes (liver cell). However, bone marrow, kidney cells, macrophages, lymphocytes and granulocytes can be affected. Once the virus has entered the cell, its envelope disappears and binds to the ribosomes, starting the viral polymerase RNA replication cycle, with an average of 10 billion virons occurring per day and released into the general bloodstream and lymphatic circulation, thereby reinfecting new cells and previously infected cells. HCV is demonstrated by RNA polymerase chain reaction (PCR).

If there are more than 10 million virons per milliliter, they can be detected by PCR. Even 0.0001 milliliters of blood is sufficient for infection. Clinical and laboratory manifestations of hepatitis C hepatitis C is very rare and difficult to detect in the acute phase. Most of them become chronic, with an incubation period of 6 weeks. First and sometimes the symptoms are weakness, headache, loss of appetite, mild abdominal pain. The preicteric period is from these symptoms to the onset of jaundice and is usually 2-12 days. The icteric period is seen with the onset of jaundice and darkening of the urine. During the recovery period, the symptoms disappear.

HCV RNA and elevated liver enzymes are present for at least six months in chronic hepatitis. Patients may have no symptoms at all or may show a sharp worsening and recurrence of symptoms. Serological and virological studies help to identify hepatitis C from other hepatic viral illnesses. Typically, liver enzymes were impacted. There may be an impact on ALT, AST, GGT, ALP, Bilirubin, Prothrombin time, and platelets. 20–25% of patients will develop cirrhosis and hepatocellular cancer within 20 years. 10% of patients recover completely. Currently used medications include ribavirin and interferon. They have a number of negative impacts.

Ozone is an extremely potent oxidant, and when it is administered to the blood in escalating doses, it affects a variety of blood cells, which is how it is used to treat hepatitis C. Platelets and leukocytes may withstand oxidative stress. Recent research has revealed ozone's antiviral properties. Ozone decomposes the virus by affecting the lipid envelope, according to studies. HIV types 1 and 2 and HCV are the most susceptible. Recent research has demonstrated improvements in liver enzymes, general patient health, and HCV virus load. Technically speaking, MAHT is advised.